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KMID : 0545120180280122106
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Journal of Microbiology and Biotechnology
2018 Volume.28 No. 12 p.2106 ~ p.2112
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Cadmium-substituted concanavalin A and its trimeric complexation
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Park Yeo-Reum
Kim Da-Som
Lee Dong-Heon
Kang Hyun-Goo
Park Jung-Hee
Lee Seung-Jae
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Abstract
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Concanavalin A (ConA) interacts with carbohydrates as a lectin, and recent reports proposed its application for detecting a diversity of viruses and pathogens. Structural studies have detailed the interaction between ConA and carbohydrates and the metal coordination environment with manganese and calcium ions (Mn-Ca-ConA). In this study, ConA was crystallized with a cadmium-containing precipitant, and the refined structure indicates that Mn2+ was replaced by Cd2+ (Cd-Ca-ConA). The structural comparison with ConA demonstrates that the metal-coordinated residues of Cd-Ca-ConA, that is Glu8, Asp10, Asn14, Asp19, and His24, do not have conformational shifts, but residues for sugar binding, including Arg228, Tyr100, and Leu99, reorient their side chains, slightly. Previous studies demonstrated that excess cadmium ions can coordinate with other residues, including Glu87 and Glu183, which were not coordinated with Cd2+ in this study. The trimeric ConA in this study coordinated Cd2+ with other residues, including Asp80 and Asp82, for the complex generation. The monomers does not have specific interaction near interface regions with the other monomer, but secondary cadmium coordinated with two aspartates (Asp80 and Asp82) from monomer 1 and one aspartate (Asp16) from monomer 2. This study demonstrated that complex generation was induced via coordination with secondary Cd2+ and showed the application potential regarding the design of complex formation for specific interactions with target saccharides.
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KEYWORD
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Biotechnology of lectins, concanavalin A, cadmium substitution, metal coordination
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